Link: https://www.scielo.br/j/rsbmt/a/ZNCvwtRjTS5PYVdG9YRjchy/?lang=en
Autores: CABANA, A.L. ; MENDES, J.F. ; KLAFKE, G.B. ; BRANDOLT, T. M. ; MARTINS, A. ; MEIRELES, M. C. A. ; XAVIER, M. O. .
Periódico: SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL. REVISTA
Resumo:
INTRODUCTION: Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, the impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated.
METHODS: We studied 12 A. fumigatus isolates. Fungal conidia were serially diluted and tested for GM detection using the Platelia® Aspergillus enzyme immunoassay (EIA).
RESULTS: The conidia concentration required for an EIA-positive result was 4.8 × 103 (median).
CONCLUSIONS: This is the first study to evaluate the impact of environmental contamination on the Platelia® Aspergillus EIA assay. Only massive contamination can interfere with GM optical readings, suggesting that environmental contamination does not cause false-positive test results.
Palavras-chave: Invasive aspergillosis; Diagnosis; Platelia® Aspergillus EIA; Galactomannan; Air quality
