Molecular detection of Paracoccidioides in soil from an urban area of southern Brazil.

Link: https://www.scielo.br/j/rsbmt/a/yfvWRKLMsPgg8CbkpGDfPJN/?lang=en

Autores: MENDES, J. F. ; POESTER, V. R. ; VON GROLL, A ; MEIRELES, M. C. A. ; XAVIER, M. O.

Periódico: SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL. REVISTA

Resumo:

INTRODUCTION:  Previous studies that detected Paracoccidioides spp. DNA in soil taken from rural areas have shown this to be a valuable tool for ecological and epidemiological studies. This study reports the detection of Paracoccidioides spp. DNA in soil samples from an urban area of southern Brazil.

METHODS:  Sixteen soil samples were submitted to nested-PCR and the amplicons of a representative number of positive samples were sequenced.

RESULTS:  Paracoccidioides spp. DNA was found in 44% of samples. Four DNA amplicons were sequenced, showing 100% homology with P. brasiliensis.

CONCLUSIONS:  The southern Brazilian urban population is commonly exposed to the Paracoccidioides fungus.

Palavras-chave: Paracoccidioidomycosis; Paracoccidioides brasiliensis; Environment