Autores: WILOT, L. C. ; SEVERO, C. B. ; STEVENS, DAVID A ; POESTER, V. R. ; XAVIER, M. O. .
Periódico: REVISTA IBEROAMERICANA DE MICOLOGIA
Resumo: Background: Early diagnosis of candidemia is critical for the correct management and treatment of patients. Aims: To test the efficacy of different blood culture bottles in the growth of Candida strains. Methods: We compared the performance of BD BACTEC™ Plus Aerobic/F (Aero) culture bottles with the specific BD BACTEC™ Mycosis IC/F Lytic (Myco) culture bottles using the BD BACTEC™ FX 40 automated blood culture system to determine the mean time-to-detection (TTD) in Candida species. One isolate each of six Candida species was inoculated into blood culture bottles (final concentration, 1–5 CFU ml−1) and incubated at 37 °C until automated growth detection. Results: Candida albicans and Nakaseomyces glabratus (Candida glabrata) were detected earlier in the specific culture bottle, whereas Candida tropicalis was detected earlier in the nonspecific bottle; Candida parapsilosis, Pichia kudriavzevii (Candida krusei), and Meyerozyma guilliermondii (Candida guilliermondii) presented similar TTD in both bottles. Conclusions: Our study suggests the suitability of using both bottles in clinical laboratories for a faster diagnosis and prompt starting of any treatment.
